Genetic engineering
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چکیده
BglII 5’...A_GATCT...3’ (5’overhang) BamHI 5’...G_GATCC...3’ (5’overhang); notice: MboI and Sau3AI = 5’..._GATC...3’ EcoRI 5’...G_AATTC...3’ (5’ overhang) HpaII 5’...C_CGG...3’ (5’ overhang); notice: CpG methylation blocked HindIII 5’...A_AGCTT...3’ (5’ overhang) KpnI 5’...GGTAC_C...3’ (3’ overhang) NcoI 5’...C_CATGG...3’ (5’ overhang); notice: Kozak sequence (RCC)ACCATGG NotI 5’...GC_GGCCGC...3’ (5’ overhang); notice: CpG methylation blocked PstI 5’...CTGCA_G...3’ (3’ overhang) SacI 5’...GAGCT_C...3’ (3’ overhang) SalI 5’...G_TCGAC...3’ (3’ overhang); notice: compatible with XhoI XhoI 5’...C_TCGAG...3’ (5’ overhang); notice: CpG me impaired, compatible with SalI XmnI 5’...GAANN_NNTTC...3’ (blunt end)
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